The major challenge of allergy diagnosis lies in the development of accessible and
reliable diagnostics allowing correct prediction of the clinical outcome following
exposure to the offending allergen(s) and cross-reactive structures. Since the late
nineties, evidence has accumulated that flow-assisted analysis and quantification
of ex vivo–activated basophils (according to the basophil activation test [BAT]) might meet
this requirement for different IgE-dependent allergies and particular forms of autoimmune
urticaria. Other so-called nondiagnostic applications of the BAT involve therapeutic
monitoring, follow-up of natural histories, and identification of allergenic recognition
sites. However, it has also become clear that appropriate use of the BAT necessitates
knowledge about degranulation metrics and guidance to guarantee correct execution
and interpretation of the results. Here, we have reviewed the most relevant applications
and limitations of the BAT. Some personal statements and views about its perspectives
are made.
Key words
Abbreviations used:
AIT (Allergen specific immunotherapy), BAT (Basophil activation test), Can s (Cannabis sativa), CCD (Cross-reactive carbohydrate determinant), CRD (Component resolved diagnosis), DPT (Drug provocation test), FCM (Flow cytometry), HVA (Hymenoptera venom allergy), IDHR (Immediate drug hypersensitivity reaction), MC (Mast cell), MRGPRX2 (Mas-related G protein–coupled receptor X2), sIgE (Specific IgE), STAT5 (Signal transducer and activator of transcription 5), ST (Skin test), TG-ROC (2-Graph receiver operating characteristic), VIT (Venom immunotherapy)To read this article in full you will need to make a payment
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Article Info
Publication History
Published online: November 01, 2020
Accepted:
October 20,
2020
Received in revised form:
October 14,
2020
Received:
June 13,
2020
Footnotes
D.E. is a senior clinical researcher of the Research Foundation Flanders (grant 1800614N ). Vito Sabato is a senior clinical researcher of the Research Foundation Flanders (grant 1804518N ).
Disclosure of potential conflict of interest: The authors declare that they have no relevant conflicts of interest.
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© 2020 American Academy of Allergy, Asthma & Immunology
