The Journal of Allergy and Clinical Immunology
Volume 97, Issue 1 , Pages 65-73 , January 1996

Food allergy to honey: Pollen or bee products? Characterization of allergenic proteins in honey by means of immunoblotting

Received 9 June 1994 ,Revised 20 March 1995 ,Accepted 24 March 1995.

References 

  1. Zander E, Maurizio A. Der Honig. Herkunft, Gewinnung, Eigenschaften und Untersuchung des Honigs. 2nd ed. Stuttgart: Verlag Eugen Ulmer; 1975;
  2. Stanley RG, Linskens HF. Pollen. Biology, biochemistry, management. Berlin: Springer Verlag; 1974;
  3. Hausen BM, Wollenweber E, Senff H, Post B. Propolis allergy (I) origin, properties, usage and new literature review. Contact Dermatitis. 1987;17:163–170
  4. Stüwe HT, Bruhn G, König WA, Hansen BM. The synthesis of caffeic acid esters, a new group of naturally occurring allergens. Naturwissenschaften. 1989;76:426–427
  5. Helbling A, Peter C, Berchtold E, Bogdanov S, Müller U. Allergy to honey: Relation to pollen and honey bee allergy. Allergy. 1992;47:41–49
  6. Hofer T, Wüthrich B. Nahrungsmittelallergien II. Häufigkeit der Organ-manifestationen und der Allergie-auslösenden Nahrungsmittel. Schweiz Med Wochenschr. 1985;115:1437–1442
  7. Strem E, Stoesser A. Does honey in infant feeding cause allergy?. Ann Allergy. 1958;375–379
  8. Birnbaum J, Tafforeau M, Vervloet D, Charpin J, Charpin D. Allergy to sunflower honey associated with allergy to celery. Clin Exp Allergy. 1989;19:229–230
  9. Bousquet J, Campos J, Michel FB. Food intolerance to honey. Allergy. 1984;39:73–75
  10. Helbling A, Wüthrich B. Ein ungewöhnlicher Fall von Honigallergie. Allergologie. 1987;10:252–255
  11. Fernández C, Martín-Esteban M, Fiandor A, et al.  Analysis of cross-reactivity between sunflower pollen and other pollens of the Compositae family. J ALLERGY CLIN IMMUNOL. 1993;92:660–667
  12. Reisman R. Insect allergy. In: 3rd ed.  Middletown E,  Reid CE,  Ellis EF,  Adkinson NF,  Yunginger JW editor. Allergy: principals and practice. St. Louis: CV Mosby; 1988;p. 1345–1358
  13. Bradford MM. A rapid and sensitive method for the quantitation of microgramm quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72:248–254
  14. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;227:680–685
  15. Jarolim E, Rumpold H, Endler AT, et al.  IgE and IgG antibodies of patients with allergy to birch pollen as tools to define the allergen profile of Betula verrucosa. Allergy. 1989;44:385–395
  16. Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci. 1979;76:4350–4354
  17. Ornstein L. Disc electrophoresis I. Background and theory. Ann NY Acad Sci. 1964;121:321
  18. Davis BJ. Disc electrophoresis II. Method and applications to human serum proteins. Ann NY Acad Sci. 1964;121:404
  19. Jarolim E, Tejkl M, Rohac M, et al.  Monoclonal antibodies against birch-pollen allergens: characterisation by immunoblotting and use for single-step affinity purification of the major allergen Bet v 1. Int Arch Allergy Appl Immunol. 1989;90:54–60
  20. Ebner C, Birkner T, Valenta R, et al.  Common epitopes of birch pollen and apples: studies by Western and Northern blot. J ALLERGY CLIN IMMUNOL. 1991;88:588–594
  21. Vallier P, Dechamp C, Valenta R, Vial O, Deviller P. Purification and characterization of an allergen from celery immunochemically related to an allergen present in several other plant species. Identification as a profilin. Clin Exp Allergy. 1992;22:774–782
  22. Valenta R, Duchêne M, Ebner C, et al.  Profilins constitute a novel family of functional plant pan-allergens. J Exp Med. 1992;2:377–385
  23. Valenta R, Duchêne , Pettenburger K, et al.  Identification of profilin as a novel pollen allergen: IgE autoreactivity in sensitized individuals. Science. 1991;253:557–560
  24. Kemeny DM, Harries MG, Youlten LJ, Mackenzie-Mills M, Lessof MH. Antibodies to purified bee venom proteins and peptides I. Development of a highly specific RAST for bee venom antigens and its application to bee sting allergy. J ALLERGY CLIN IMMUNOL. 1983;71:505–514
  25. Aalberse RC, Koshte V, Clemens JGJ. Immunoglobulin E antibodies that cross-react with vegetable foods, pollen and Hymenoptera venom. J ALLERGY CLIN IMMUNOL. 1981;68:356–365

 From the aInstitute of General and Experimental Pathology, AKH, University of Vienna, Austria; the bInstitute of Bee Research, Bad Vöslau, Austria; and the cAllergy Clinic Reumannplatz, Vienna, Austria.

☆☆ Supported by grant SO 6704-MED and 6707-MED from the “Fonds zur Förderung der Wissenschaftlichen Forschung,” Austria.

 Reprint requests: Christof Ebner, MD, Institute of General and Experimental Pathology, AKH, University of Vienna, Währinger Gürtel 18-20, A-1090 Vienna, Austria.

★★ 0091-6749/96 $5.00 + 0 1/1/65031

PII: S0091-6749(96)70284-1

The Journal of Allergy and Clinical Immunology
Volume 97, Issue 1 , Pages 65-73 , January 1996