Food allergy to honey: Pollen or bee products? Characterization of allergenic proteins in honey by means of immunoblotting

Honey extracts: Coomassie brilliant blue R–stained 12% SDS-polyacrylamide gel shows the separation of four different types of honey: lane 1, FO; lane 2, CH; lane 3, SF; lane 4, LO; lane M, molecular weight marker (Amersham International, U.K.).

Autoradiograph of IgE-immunoblot analysis of SF extract with sera from patients of group I. Lane N, Normal human serum pool; lane B, buffer control.

Autoradiograph of IgE-immunoblot analysis of extracts of SF, LO, CH, and FO with serum pools from patients of group I. Lane 1, pool A; lane 2, pool B; lane 3, pool C; lane B, buffer control.

A, Autoradiograph of IgE immunoblot analysis of honeybee head (CA) extract with sera from group I. B, Autoradiograph of IgE immunoblot analysis of bee venom sac (BV) extract with sera from group I. Lane N, Normal human serum pool; lane B, buffer control.

A, Autoradiograph of IgE immunoblot analysis of honeybee head (CA) extract with sera from group I. B, Autoradiograph of IgE immunoblot analysis of bee venom sac (BV) extract with sera from group I. Lane N, Normal human serum pool; lane B, buffer control.

Autoradiograph of IgE immunoblot analysis of extracts of bee venom sacs (BV), SF, and LO, CH, and FO with sera from group II. Lane N, Normal human serum pool; lane B, buffer control.

Autoradiograph of IgE immunoblot analysis of extracts of bee venom sacs (BV), SF, and LO, CH, and FO with sera from group II. Lane N, Normal human serum pool; lane B, buffer control.

Autoradiograph of IgE immunoblot analysis of extracts of bee venom sacs (BV), SF, and LO, CH, and FO with sera from group II. Lane N, Normal human serum pool; lane B, buffer control.

Autoradiograph of IgE inhibition experiments performed with SF and CA extracts, respectively. Serum pool A, pool B, and pool C were preincubated with 0 μg and 300 μg of CA extract (SF; lanes 1, 2, 4, 5, 7, 8), and 80 μg of SF extract (SF; lanes 3, 6, 9). Nitrocellulose-blotted sunflower honey proteins were incubated with this solution, and immunoblotting was performed. Serum pool B was preincubated with 0 μg, 40 μg, and 80 μg of SF extract (CA; lanes 1 to 3) and 300 μg of CA extract (CA; lane 4). Thereafter nitrocellulose-blotted honeybee head proteins were incubated with this solution, and immunoblotting was performed. Serum pool X was preincubated with 0 μg, 5 μg, 25 μg, and 125 μg of SF extract (BV; lanes 1 through 4) and 100 μg of BV extract (BV; lane 5). Nitrocellulose-blotted honeybee sac proteins were incubated with this solution, and immunoblotting was performed. Lane B, buffer control.

Autoradiograph of IgE inhibition experiments performed with SF and CA extracts, respectively. Serum pool A, pool B, and pool C were preincubated with 0 μg and 300 μg of CA extract (SF; lanes 1, 2, 4, 5, 7, 8), and 80 μg of SF extract (SF; lanes 3, 6, 9). Nitrocellulose-blotted sunflower honey proteins were incubated with this solution, and immunoblotting was performed. Serum pool B was preincubated with 0 μg, 40 μg, and 80 μg of SF extract (CA; lanes 1 to 3) and 300 μg of CA extract (CA; lane 4). Thereafter nitrocellulose-blotted honeybee head proteins were incubated with this solution, and immunoblotting was performed. Serum pool X was preincubated with 0 μg, 5 μg, 25 μg, and 125 μg of SF extract (BV; lanes 1 through 4) and 100 μg of BV extract (BV; lane 5). Nitrocellulose-blotted honeybee sac proteins were incubated with this solution, and immunoblotting was performed. Lane B, buffer control.

Autoradiograph of IgE inhibition experiments performed with SF and CA extracts, respectively. Serum pool A, pool B, and pool C were preincubated with 0 μg and 300 μg of CA extract (SF; lanes 1, 2, 4, 5, 7, 8), and 80 μg of SF extract (SF; lanes 3, 6, 9). Nitrocellulose-blotted sunflower honey proteins were incubated with this solution, and immunoblotting was performed. Serum pool B was preincubated with 0 μg, 40 μg, and 80 μg of SF extract (CA; lanes 1 to 3) and 300 μg of CA extract (CA; lane 4). Thereafter nitrocellulose-blotted honeybee head proteins were incubated with this solution, and immunoblotting was performed. Serum pool X was preincubated with 0 μg, 5 μg, 25 μg, and 125 μg of SF extract (BV; lanes 1 through 4) and 100 μg of BV extract (BV; lane 5). Nitrocellulose-blotted honeybee sac proteins were incubated with this solution, and immunoblotting was performed. Lane B, buffer control.

Autoradiograph of IgE inhibition experiments performed with SF and CA extracts, respectively. Serum pool A, pool B, and pool C were preincubated with 0 μg and 300 μg of CA extract (SF; lanes 1, 2, 4, 5, 7, 8), and 80 μg of SF extract (SF; lanes 3, 6, 9). Nitrocellulose-blotted sunflower honey proteins were incubated with this solution, and immunoblotting was performed. Serum pool B was preincubated with 0 μg, 40 μg, and 80 μg of SF extract (CA; lanes 1 to 3) and 300 μg of CA extract (CA; lane 4). Thereafter nitrocellulose-blotted honeybee head proteins were incubated with this solution, and immunoblotting was performed. Serum pool X was preincubated with 0 μg, 5 μg, 25 μg, and 125 μg of SF extract (BV; lanes 1 through 4) and 100 μg of BV extract (BV; lane 5). Nitrocellulose-blotted honeybee sac proteins were incubated with this solution, and immunoblotting was performed. Lane B, buffer control.

Autoradiograph of IgE inhibition experiments performed with SF and CA extracts, respectively. Serum pool A, pool B, and pool C were preincubated with 0 μg and 300 μg of CA extract (SF; lanes 1, 2, 4, 5, 7, 8), and 80 μg of SF extract (SF; lanes 3, 6, 9). Nitrocellulose-blotted sunflower honey proteins were incubated with this solution, and immunoblotting was performed. Serum pool B was preincubated with 0 μg, 40 μg, and 80 μg of SF extract (CA; lanes 1 to 3) and 300 μg of CA extract (CA; lane 4). Thereafter nitrocellulose-blotted honeybee head proteins were incubated with this solution, and immunoblotting was performed. Serum pool X was preincubated with 0 μg, 5 μg, 25 μg, and 125 μg of SF extract (BV; lanes 1 through 4) and 100 μg of BV extract (BV; lane 5). Nitrocellulose-blotted honeybee sac proteins were incubated with this solution, and immunoblotting was performed. Lane B, buffer control.