The Journal of Allergy and Clinical Immunology
Volume 125, Issue 5 , Pages 995-1000 , May 2010

Decreased serum vitamin D levels in children with asthma are associated with increased corticosteroid use

  • Daniel A. Searing, MD

      Affiliations

    • Division of Allergy and Immunology, Department of Pediatrics, National Jewish Health, Denver, Colo
    • ,
  • Yong Zhang, PhD

      Affiliations

    • Division of Allergy and Immunology, Department of Pediatrics, National Jewish Health, Denver, Colo
    • ,
  • James R. Murphy, PhD

      Affiliations

    • Division of Biostatistics and Bioinformatics, National Jewish Health, Denver, Colo
    • Department of Preventive Medicine and Biometrics, University of Colorado Denver, Aurora, Colo
    • ,
  • Pia J. Hauk, MD

      Affiliations

    • Division of Allergy and Immunology, Department of Pediatrics, National Jewish Health, Denver, Colo
    • Department of Pediatrics, University of Colorado Denver, Aurora, Colo
    • ,
  • Elena Goleva, PhD

      Affiliations

    • Division of Allergy and Immunology, Department of Pediatrics, National Jewish Health, Denver, Colo
    • ,
  • Donald Y.M. Leung, MD, PhD

      Affiliations

    • Division of Allergy and Immunology, Department of Pediatrics, National Jewish Health, Denver, Colo
    • Department of Pediatrics, University of Colorado Denver, Aurora, Colo
    • Corresponding Author InformationReprint requests: Donald Y. M. Leung, MD, PhD, Division of Pediatric Allergy and Immunology, National Jewish Health, 1400 Jackson St, K926i, Denver, CO 80206.

Received 23 December 2009 ,Revised 8 February 2010 ,Accepted 15 March 2010.

  • Image Result

    Distribution of VitD levels. VitD levels are expressed as serum 25-hydroxyvitamin D. The median value was 31 ng/mL. Forty-seven percent of asthmatic patients had insufficient levels of VitD (<30 ng/mL

    Distribution of VitD levels. VitD levels are expressed as serum 25-hydroxyvitamin D. The median value was 31 ng/mL. Forty-seven percent of asthmatic patients had insufficient levels of VitD (<30 ng/mL).

  • Image Result
    VitD (VD)–potentiated DEX-mediated transactivation in human PBMCs enhances DEX induction of MKP1 (A) and IL10 (B) mRNA. PBMCs from patients with asthma were cultured with 10 nmol/L VitD or media alone

    VitD (VD)–potentiated DEX-mediated transactivation in human PBMCs enhances DEX induction of MKP1 (A) and IL10 (B) mRNA. PBMCs from patients with asthma were cultured with 10 nmol/L VitD or media alone for 24 hours and supplemented with 10 or 100 nmol/L DEX for the last 3 hours of culture. MKP1 mRNA levels were detected by means of real-time PCR and were normalized to actin mRNA. Values represent means ± SEMs (n = 11).

  • Image Result
    VitD augments DEX's effect on TSST-1–stimulated lymphocyte proliferation in human PBMCs. PBMCs from healthy control subjects were treated with DEX and VitD, as indicated in the Methods section. Tritia

    VitD augments DEX's effect on TSST-1–stimulated lymphocyte proliferation in human PBMCs. PBMCs from healthy control subjects were treated with DEX and VitD, as indicated in the Methods section. Tritiated thymidine was added to the medium 18 hours before collecting the cells. Cell division was estimated based on tritiated thymidine incorporation (n = 4).

 Supported by National Institutes of Health grants AI070140 and HL37260.

 Disclosure of potential conflict of interest: The authors have declared that they have no conflict of interest.

PII: S0091-6749(10)00505-1

doi: 10.1016/j.jaci.2010.03.008

The Journal of Allergy and Clinical Immunology
Volume 125, Issue 5 , Pages 995-1000 , May 2010

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