The Journal of Allergy and Clinical Immunology
Volume 125, Issue 1 , Pages 39-49 , January 2010

Secreted virulence factor comparison between methicillin-resistant and methicillin-sensitive Staphylococcus aureus, and its relevance to atopic dermatitis

  • Patrick M. Schlievert, PhD

      Affiliations

    • Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minn
    • Corresponding Author InformationReprint requests: Patrick M. Schlievert, PhD, Department of Microbiology, Medical School, University of Minnesota, 420 Delaware Street SE, Minneapolis, MN 55455.
    • ,
  • Kristi L. Strandberg, PhD

      Affiliations

    • Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minn
    • ,
  • Ying-Chi Lin, MS

      Affiliations

    • Department of Experimental and Clinical Pharmacology, College of Pharmacy, Minneapolis, Minn
    • ,
  • Marnie L. Peterson, PharmD, PhD

      Affiliations

    • Department of Experimental and Clinical Pharmacology, College of Pharmacy, Minneapolis, Minn
    • ,
  • Donald Y.M. Leung, MD, PhD

      Affiliations

    • Department of Pediatrics, National Jewish Health, Denver, Colo

Received 27 August 2009 ,Revised 23 October 2009 ,Accepted 26 October 2009.

  • Image Result

    Virulence factor production by S aureus. The organism produces cell surface virulence factors (MSCRAMMs) during the exponential phase and exoproteins and exopolysaccharides during the postexponential/

    Virulence factor production by S aureus. The organism produces cell surface virulence factors (MSCRAMMs) during the exponential phase and exoproteins and exopolysaccharides during the postexponential/stationary phase.

  • Image Result
    Model for the activation of CD4+T cells and macrophages by the superantigen (SAg) SEB compared with antigenic peptide activation of the same cells. Ribbon diagrams of TCRs, MHC II molecules, and SEB a

    Model for the activation of CD4+T cells and macrophages by the superantigen (SAg) SEB compared with antigenic peptide activation of the same cells. Ribbon diagrams of TCRs, MHC II molecules, and SEB adapted from previously published studies.

  • Image Result
    Three-dimensional cartoon diagram of the staphylococcal α-toxin homo-chain heptamer adapted from Gouaux et al.75 γ-Toxins and PVL structures are similar except the heptamers are composed of combinatio

    Three-dimensional cartoon diagram of the staphylococcal α-toxin homo-chain heptamer adapted from Gouaux et al.75 γ-Toxins and PVL structures are similar except the heptamers are composed of combinations of F (Fast) and S (Slow) peptides.72 Gray is α-toxin monomer (A); multicolored is α-toxin heptamer (B).

  • Image Result
    Secreted protein profiles of CA-MSSA USA200 strains CDC587 and MNPE and CA-MRSA USA200 strain MNWH. All organisms were cultured to stationary phase, cells removed by centrifugation, sterile supernates

    Secreted protein profiles of CA-MSSA USA200 strains CDC587 and MNPE and CA-MRSA USA200 strain MNWH. All organisms were cultured to stationary phase, cells removed by centrifugation, sterile supernates concentrated 10-fold, SDS-PAGE performed, and gel stained with Coomassie brilliant blue R-250.

  • Image Result
    Carotenoid pigment production by CA-MSSA USA200 strains CDC587 and MNPE, and CA-MRSA USA200 strain MNWH. Organisms were cultured to the stationary phase, and cells were pelleted by centrifugation.

    Carotenoid pigment production by CA-MSSA USA200 strains CDC587 and MNPE, and CA-MRSA USA200 strain MNWH. Organisms were cultured to the stationary phase, and cells were pelleted by centrifugation.

 Supported by National Institutes of Health grants R01s AI74283, AI73366, and AR41256; U54-AI57153 (Great Lakes Regional Center of Excellence in Biodefense and Emerging Infectious Diseases, where P.M.S. is a member); and contract N01 AI40029 to D.Y.M.L.

 Disclosure of potential conflict of interest: P. M. Schlievert receives research support from the NIH and has provided legal consultation/expert witness testimony in cases related to streptococcal toxic shock syndrome. M. L. Peterson receives research support from the NIH and 3M. D. Y. M. Leung receives research support from the NIH/NIAID, the NIH/NIAMS, and Novartis Pharmaceuticals. The rest of the authors have declared that they have no conflict of interest.

PII: S0091-6749(09)01605-4

doi: 10.1016/j.jaci.2009.10.039

The Journal of Allergy and Clinical Immunology
Volume 125, Issue 1 , Pages 39-49 , January 2010

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