The Journal of Allergy and Clinical Immunology
Volume 123, Issue 2 , Pages 452-458.e4 , February 2009

Soybean (Glycine max) allergy in Europe: Gly m 5 (β-conglycinin) and Gly m 6 (glycinin) are potential diagnostic markers for severe allergic reactions to soy

  • Thomas Holzhauser, PhD

      Affiliations

    • Division of Allergology, Paul-Ehrlich-Institut, Langen, Germany
    • Corresponding Author InformationReprint requests: Thomas Holzhauser, PhD, Division of Allergology, Paul-Ehrlich-Institut, Paul-Ehrlich-Strasse 51-59, D-63225 Langen, Germany.
  • ,
  • Olga Wackermann, MSc

      Affiliations

    • Division of Allergology, Paul-Ehrlich-Institut, Langen, Germany
  • ,
  • Barbara K. Ballmer-Weber, MD

      Affiliations

    • Allergy Unit, Department of Dermatology, University Hospital, Zurich, Switzerland
  • ,
  • Carsten Bindslev-Jensen, MD

      Affiliations

    • Allergy Center, Odense University Hospital, Odense, Denmark
  • ,
  • Joseph Scibilia, MD

      Affiliations

    • Allergology and Clinical Immunology Unit, Niguarda Cá Granda Hospital, Department of Medicine, Milan, Italy
  • ,
  • Lorenza Perono-Garoffo, PhD

      Affiliations

    • National Council of Research, Istituto di Science delle Produzioni Alimentari, Italy
  • ,
  • Shigeru Utsumi, PhD

      Affiliations

    • Research Institute for Food Science, Kyoto University, Kyoto, Japan
  • ,
  • Lars K. Poulsen, PhD

      Affiliations

    • Allergy Clinic, National University Hospital, Copenhagen, Denmark
  • ,
  • Stefan Vieths, PhD

      Affiliations

    • Division of Allergology, Paul-Ehrlich-Institut, Langen, Germany

Received 4 June 2008 ,Revised 10 September 2008 ,Accepted 19 September 2008.

References 

  1. Yunginger JW, Nelson DR, Squillace DL, Jones RT, Holley KE, Hyma BA, et al. Laboratory investigation of deaths due to anaphylaxis. J Forensic Sci. 1991;36:857–865
  2. Mittag D, Vieths S, Vogel L, Becker W-M, Rhis H-P, Helbling A, et al. Birch pollen-related allergy to soybean: clinical investigation and molecular characterization of allergens. J Allergy Clin Immunol. 2004;113:148–154
  3. Ballmer-Weber BK, Holzhauser T, Scibilia J, Mittag D, Zisa G, Ortolani C, et al. Clinical characteristics of soybean allergy in Europe: a double-blind placebo-controlled food challenge study. J Allergy Clin Immunol. 2007;119:1489–1496
  4. Sampson HA, Ho DG. Relationship between food-specific IgE concentrations and the risk of positive food challenges in children and adolescents. J Allergy Clin Immunol. 1997;100:444–451
  5. Niggemann B, Sielaff B, Beyer K, Binder C, Wahn U. Outcome of double-blind, placebo-controlled food challenge tests in 107 children with atopic dermatitis. Clin Exp Allergy. 1999;29:91–96
  6. Sicherer SH, Morrow EH, Sampson HA. Dose-response in double-blind, placebo-controlled oral food challenges in children with atopic dermatitis. J Allergy Clin Immunol. 2000;105:582–586
  7. Besler M, Helm R, Ogawa T. Allergen data collection-update: soybean. Internet Symposium on Food Allergens. 2000;2(suppl 3):1–35Available at: http://www.food-allergens.deAccessed October 29, 2008
  8. Sicherer SH, Sampson HA, Burks AW. Peanut and soy allergy: a clinical and therapeutic dilemma. Allergy. 2000;55:515–521
  9. Hourihane JO'B, Kilburn SA, Nordlee JA, Hefle SL, Taylor SL, Warner JO. An evaluation of the sensitivity of subjects with peanut allergy to very low doses of peanut protein: a randomized, double-blind, placebo-controlled food challenge study. J Allergy Clin Immunol. 1997;100:596–600
  10. Wensing M, Penninks AH, Hefle SL, Koppelman SJ, Bruijnzeel-Koomen CA, Knulst AC. The distribution of individual threshold doses eliciting allergic reactions in a population with peanut allergy. J Allergy Clin Immunol. 2002;110:915–920
  11. Moroz LA, Yang WH. Kunitz soybean trypsin inhibitor: a specific allergen in food anaphylaxis. N Engl J Med. 1980;302:1126–1128
  12. Burks AW, Cockrell G, Connaughton C, Guin J, Allen W, Helm RM. Identification of peanut agglutinin and soybean trypsin inhibitor as minor legume allergens. Int Arch Allergy Immunol. 1994;105:143–149
  13. Gu X, Beardslee T, Zeece M, Sarath G, Markwell J. Identification of IgE-binding proteins in soy lecithin. Int Arch Allergy Immunol. 2001;126:218–225
  14. Ogawa T, Bando N, Tsuji H, Okajima H, Nishikawa K, Sasaoka K. Investigation of the IgE-binding proteins in soybean by immunoblotting with the sera of the soybean-sensitive patients with atopic dermatitis. J Nutr Sci Vitaminol. 1991;37:555–565
  15. Helm RM, Cockrell G, Herman E, Burks AW, Sampson HA, Bannon GA. Cellular and molecular characterization of a major soybean allergen. Int Arch Allergy Immunol. 1998;117:29–37
  16. Helm RM, Cockrell G, Connaughton C, West CM, Herman E, Sampson HA, et al. Mutational analysis of the IgE-binding epitopes of P34/Gly m Bd 30k. J Allergy Clin Immunol. 2000;105:378–384
  17. Ogawa T, Bando N, Tsuji H, Nishikawa K, Kitamura K. α-Subunit of β-conglycinin, an allergenic protein recognized by IgE antibodies of soybean-sensitive patients with atopic dermatitis. Biosci Biotech Biochem. 1995;59:831–833
  18. Beardslee TA, Zeece MG, Sarath G, Markwell JP. Soybean G1 acidic chain shares IgE epitopes with peanut allergen Ara h 3. Int Arch Allergy Immunol. 2000;123:299–307
  19. Helm RM, Cockrell G, Connaughton C, Sampson HA, Bannon GA, Beilinson V, et al. A soybean G2 glycinin allergen. Int Arch Allergy Immunol. 2000;123:205–212
  20. Rozenfeld P, Docena GH, Anon MC, Fossati CA. Detection and identification of a soy protein component that cross-reacts with caseins from cow's milk. Clin Exp Immunol. 2002;130:49–58
  21. Lin J, Shewry PR, Archer DB, Beyer K, Niggemann B, Haas H, et al. The potential allergenicity of two 2S albumins from soybean (Glycine max L.): a protein microarray approach. Int Arch Allergy Immunol. 2006;141:91–102
  22. Maruyama N, Katsube T, Wada Y, Oh MH, Barba De La Rosa AP, Okuda E, et al. The roles of the N-linked glycans and extension regions of soybean beta-conglycinin in folding, assembly and structural features. Eur J Biochem. 1998;258:854–862
  23. Prak K, Nakatani K, Katsube-Tanaka T, Adachi M, Maruyama N, Utsumi S. Structure-function relationships of soybean proglycinins at subunit levels. J Agric Food Chem. 2005;53:3650–3657
  24. Heukeshoven J, Dernick R. Simplified method for silver staining of proteins in polyacrylamide gels and the mechanism of silver staining. Electrophoresis. 1985;6:103–112
  25. Hoff M, Ballmer-Weber BK, Niggemann B, Cistero-Bahima A, San Miguel-Moncin M, Conti A, et al. Molecular cloning and immunological characterisation of potential allergens from the mould Fusarium culmorum. Mol Immunol. 2003;39:965–975
  26. Reese G, Viebranz J, Leong-Kee SM, Plante M, Lauer I, Randow S, et al. Reduced allergenic potency of VR9-1, a mutant of the major shrimp allergen Pen a 1 (tropomyosin). J Immunol. 2005;175:8354–8364
  27. Hellmann U, Wernstedt C, Gonez J, Heldin CH. Improvement of an “in-gel” digestion procedure for the micropreparation of internal protein fragments for amino acid sequencing. Anal Biochem. 1995;224:451–455
  28. Conti A, Giuffrida MG, Hoffmann-Sommergruber K, Wagner S, Amato S, Mistrello G, et al. Identification of latex UDP glucose pyrophosphorylase (Hev b UDPGP) as a novel cause of latex fruit allergy syndrome. Eur Ann Allergy Clin Immunol. 2007;39:116–118
  29. Holzhauser T, Vieths S. Indirect competitive ELISA for determination of traces of peanut (Arachis Hypogaea L.) protein in complex food matrixes. J Agric Food Chem. 1999;47:603–611
  30. Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 1997;25:3389–3402
  31. Liu X, Feng J, Xu ZR, Wang YZ, Liu JX. Oral allergy syndrome and anaphylactic reactions in BALB/c mice caused by soybean glycinin and β-conglycinin. Clin Exp Allergy. 2008;38:350–356
  32. Kleine-Tebbe J, Vogel L, Crowell DN, Haustein UF, Vieths S. Severe oral allergy syndrome and anaphylactic reactions caused by a Bet v 1-related PR-10 protein in soybean, SAM22. J Allergy Clin Immunol. 2002;110:797–804

 Supported by the 5th Framework Programme: Quality of Life and Management of Living Resources of the European Commission, QLK4-CT-2001-00301, and by the Swiss Federal Office for Education and Science, BBW01.0159-1.

 Disclosure of potential conflict of interest: C. Bindslev-Jensen has served as an expert witness in a patient antihistamine injury case. L. K. Poulsen has served as an expert witness for Novozymes Ltd. S. Vieths is an associate at the Institute for Product Quality Berlin; has received honoraria from Phadia and the Food Allergy Resource and Research Program; has received research support from the European Union, the Germany Research Society, the Research Fund of the German Food Industry, Monsanto Co, and the European Directorate for the Quality of Medicine and Health Care; and has served as a member of the European Academy of Allergy and Clinical Immunology, the International Union of Immunological Diseases, the European Agency for the Evaluation of Medicinal Products, the European Pharmacopoeia Commission, the International Life Sciences Institute Health and Environmental Science Institute, the European Committee for Standardization, and Deutsche Gesellschaft fur Allergie und Klinische Immunologie. The rest of the authors have declared that they have no conflict of interest.

PII: S0091-6749(08)01729-6

doi: 10.1016/j.jaci.2008.09.034

The Journal of Allergy and Clinical Immunology
Volume 123, Issue 2 , Pages 452-458.e4 , February 2009