Soybean (Glycine max) allergy in Europe: Gly m 5 (β-conglycinin) and Gly m 6 (glycinin) are potential diagnostic markers for severe allergic reactions to soy

IgE-immunoblot analysis of a soybean extract. All lanes except for 28^∗ and P were detected with peroxidase mediated chemiluminescence (lane C: buffer control; lane N: nonatopic control serum pool [n = 3]; lanes 1, 3, 14, 18, 21, 30, and 28: sera from subjects with soybean allergy summarized in Table I; lane 28^∗: patient 28 detected with phosphatase and precipitating dye; lane P: silver-stained total soybean protein).

Purity testing of the individual Gly m 5 (β-conglycinin) subunits β (Gly m 5.03), α (Gly m 5.01), and α′ (Gly m 5.02) from natural soybean source in SDS-PAGE and silver staining (lanes β, α, and α′), and verification of the IgE reactivity of all Gly m 5 subunits by immunoblot analysis with selected soy allergic sera (lane C: buffer control; N: nonatopic control serum pool [n = 3]; lanes 5, 14, 18, 19, and 20: subjects with soybean allergy).

A, Individual subunits of Gly m 6 by total protein staining of Western blots with Ponceau S (lanes: G1, Gly m 6.01, G2, Gly m 6.02; G3, Gly m 6.03; G4, Gly m 6.04; G5, Gly m 6.05) and verification of IgE-reactivity of all Gly m 6 subunits (double lane) with patient 28 (N: nonatopic serum pool). B, IgE immunoblot analysis of equal amounts (wt/wt) of the Gly m 6 subunits with selected patients' sera (lane C: buffer control; N: nonatopic control serum pool [n = 3]; lanes 1, 12, 14, 19, and 21: subjects with soybean allergy). The arrows indicate the position of the 5 proglycinin subunits according to molecular weight.