The Journal of Allergy and Clinical Immunology
Volume 123, Issue 1 , Pages 187-194 , January 2009

Allergenicity and antigenicity of fenugreek (Trigonella foenum-graecum) proteins in foods

  • Christiane Kruse Fæste, PhD

      Affiliations

    • National Veterinary Institute, Rikshospitalet University Hospital, Oslo, Norway
    • Corresponding Author InformationReprint requests: Christiane Kruse Fæste, PhD, National Veterinary Institute, PO Box 750, Sentrum, N-0106 Oslo, Norway.
    • ,
  • Ellen Namork, PhD

      Affiliations

    • Norwegian Institute of Public Health, Rikshospitalet University Hospital, Oslo, Norway
    • ,
  • Helene Lindvik, MMSc

      Affiliations

    • Voksentoppen Children's Center for Asthma and Allergy, Rikshospitalet University Hospital, Oslo, Norway

Received 1 April 2008 ,Revised 20 August 2008 ,Accepted 4 September 2008.

  • Image Result

    Coomassie-stained SDS-PAGE of legume extracts. M1, SeeBluePlus2 prestained molecular weight marker; F, fenugreek; 1, fenugreek; 2, peanut; 3, lupin; 4, pea; 5, soy; 6, bean; 7, lentil; 8, chickpea; 9,

    Coomassie-stained SDS-PAGE of legume extracts. M1, SeeBluePlus2 prestained molecular weight marker; F, fenugreek; 1, fenugreek; 2, peanut; 3, lupin; 4, pea; 5, soy; 6, bean; 7, lentil; 8, chickpea; 9, curry; 10, tikka masala; M2, Mark12 molecular weight marker. Protein sizes (kd) are indicated on the sides of the gel.

  • Image Result
    Immunoblot using sera from fenugreek-sensitized patients (Table I). A, Fenugreek. B, Peanut. M1, Marker (kd).

    Immunoblot using sera from fenugreek-sensitized patients (Table I). A, Fenugreek. B, Peanut. M1, Marker (kd).

  • Image Result
    Immunoblot using sera from patients with fenugreek allergy (Table II). A, Patient A. B, Patient B. 1, Fenugreek; 2, peanut; 3, lupin; 4, pea; 5, soy; 6, bean; 7, lentil; 8, chickpea; 9, curry; 10, tan

    Immunoblot using sera from patients with fenugreek allergy (Table II). A, Patient A. B, Patient B. 1, Fenugreek; 2, peanut; 3, lupin; 4, pea; 5, soy; 6, bean; 7, lentil; 8, chickpea; 9, curry; 10, tandoori masala; 11, cinnamon; 12, Indian tikka masala; 13, naan authentic; M1, Marker (kd).

  • Image Result
    Fenugreek in food products. Immunoblot using polyclonal rabbit antifenugreek antibody. 1-32, Food products in the same order as in Table III. M, M1 Marker (kd).

    Fenugreek in food products. Immunoblot using polyclonal rabbit antifenugreek antibody. 1-32, Food products in the same order as in Table III. M, M1 Marker (kd).

  • Image Result
    A and B, Allergenicity of fenugreek-containing foods. Immunoblot using sera from patients differently sensitized to peanut and fenugreek. 1-32, Food products in the same order as in Table III. M, M1 m

    A and B, Allergenicity of fenugreek-containing foods. Immunoblot using sera from patients differently sensitized to peanut and fenugreek. 1-32, Food products in the same order as in Table III. M, M1 marker (kd).

 Supported by the Norwegian Research Council.

 Disclosure of potential conflict of interest: The authors have declared that they have no conflict of interest.

PII: S0091-6749(08)01673-4

doi: 10.1016/j.jaci.2008.09.012

The Journal of Allergy and Clinical Immunology
Volume 123, Issue 1 , Pages 187-194 , January 2009

Article Tools

* Image Usage & Resolution