Lung dendritic cells are stimulated by ultrafine particles and play a key role in particle adjuvant activity

Background

The adjuvant activity of air pollution particles on allergic airway sensitization is well known, but the cellular mechanisms underlying this adjuvant potential are not clear.

Objective

We sough to study the role of dendritic cells and the costimulatory molecules CD80 and CD86 in the adjuvant activity of ultrafine carbon black particles (CBP).

Methods

The proliferation of CFSE-labeled DO11.10 CD4 cells was studied after intranasal exposure to particles and ovalbumin (OVA). Next the frequency of myeloid dendritic cells (mDCs) and plasmacytoid dendritic cells and their expression of CD80 and CD86 were studied in the peribronchial lymph nodes (PBLNs). The expression of costimulatory molecules was also studied on bone marrow–derived mDCs after exposure to CBPs in vitro, and the importance of costimulation in CBP adjuvant activity was assessed by using CD80/CD86-deficient mice or cytotoxic T lymphocyte–associated antigen 4 (CTLA4)-Ig in vivo.

Results

Our data show that CBPs plus OVA caused proliferation of DO11.10 CD4 cells and high levels of cytokine production in the PBLNs. Furthermore, the combined CBP plus OVA exposure increased the number of mDCs and expression of costimulatory molecules in the PBLNs. In addition, CBPs upregulated the expression of CD80/CD86 molecules on dendritic cells in vitro, which are necessary for the particle adjuvant effects in vivo.

Conclusion

Together this study shows the importance of dendritic cells and costimulation in particle adjuvant activity. Furthermore, we show for the first time that CBPs can also directly induce maturation of dendritic cells.

Key words: Dendritic cells, costimulation, allergic inflammation, airways, particulate matter, ultrafine particles

Abbreviations used: CBP, Carbon black particles, CFSE, 5- (and 6-) Carboxyfluorescein diacetate succinimidyl ester, CLN, Cervical lymph node, CTLA, Cytotoxic T lymphocyte–associated antigen, DEP, Diesel exhaust particle, mDC, Myeloid dendritic cell, OVA, Ovalbumin, PBLN, Peribronchial lymph node, pDC, Plasmacytoid dendritic cell, SCLN, Superficial cervical lymph node