The Journal of Allergy and Clinical Immunology
Volume 120, Issue 6 , Pages 1301-1307 , December 2007

Augmented epithelial endothelin-1 expression in refractory asthma

  • Sophie Pégorier, PhD

      Affiliations

    • Inserm U700, Paris, and Université Paris 7, Faculté de Médecine Denis Diderot, site Xavier Bichat, Paris, France
    • ,
  • Nassim Arouche, MSc

      Affiliations

    • Inserm U700, Paris, and Université Paris 7, Faculté de Médecine Denis Diderot, site Xavier Bichat, Paris, France
    • ,
  • Marie-Christine Dombret, MD

      Affiliations

    • Département de Pneumologie A, Centre Hospitalier Universitaire Bichat-Claude Bernard, Assistance Publique des Hôpitaux de Paris, Paris, France
    • ,
  • Michel Aubier, MD

      Affiliations

    • Inserm U700, Paris, and Université Paris 7, Faculté de Médecine Denis Diderot, site Xavier Bichat, Paris, France
    • Département de Pneumologie A, Centre Hospitalier Universitaire Bichat-Claude Bernard, Assistance Publique des Hôpitaux de Paris, Paris, France
    • ,
  • Marina Pretolani, PhD

      Affiliations

    • Inserm U700, Paris, and Université Paris 7, Faculté de Médecine Denis Diderot, site Xavier Bichat, Paris, France
    • Corresponding Author InformationReprint requests: Marina Pretolani, PhD, Inserm Unité 700, Université Paris 7, Faculté de Médecine Denis Diderot, site Xavier Bichat, 16, rue Henri Huchard, 75018 Paris (France).

Received 12 April 2007 ,Revised 12 September 2007 ,Accepted 17 September 2007.

  • Image Result

    Representative immunostaining of EDN1 in bronchial biopsy specimens from control subjects (A), a patient with mild steroid-untreated asthma (B), and patients with severe steroid-sensitive (C) and ster

    Representative immunostaining of EDN1 in bronchial biopsy specimens from control subjects (A), a patient with mild steroid-untreated asthma (B), and patients with severe steroid-sensitive (C) and steroid-refractory (D) asthma is shown. Scale bars = 250 μm. E, proportion of EDN1-positive epithelial cells per millimeter of SBM in bronchial biopsy specimens from control subjects and the 3 groups of asthmatic patients. Bars represent median values, and error bars denote interquartile ranges.

  • Image Result
    Epithelial integrity (A), SBM thickness (B), and ASM areas (C) in bronchial biopsy specimens from control subjects and asthmatic patients are shown. Bars represent median values, and error bars denote

    Epithelial integrity (A), SBM thickness (B), and ASM areas (C) in bronchial biopsy specimens from control subjects and asthmatic patients are shown. Bars represent median values, and error bars denote interquartile ranges.

  • Image Result
    Representative immunostaining of fibroblasts and myofibroblasts (propyl-4-hydroxylase–positive cells) in bronchial biopsy specimens from a control subject (A), a patient with mild steroid-untreated as

    Representative immunostaining of fibroblasts and myofibroblasts (propyl-4-hydroxylase–positive cells) in bronchial biopsy specimens from a control subject (A), a patient with mild steroid-untreated asthma (B), and patients with severe steroid-sensitive (C) and refractory (D) asthma is shown. Scale bars = 250 μm. E, Numbers of fibroblasts/myofibroblasts per millimeter of SBM in bronchial biopsy specimens from control subjects and the 3 groups of asthmatic patients. Bars represent median values, and error bars denote interquartile ranges.

 Supported in part by the “Agence Nationale de la Recherche,” Paris, France (grant no. 0012405). Sophie Pégorier was funded by the “Chancellerie des Universités de Paris en Sorbonne,” Paris, France.

 Disclosure of potential conflict of interest: The authors have declared that they have no conflict of interest.

PII: S0091-6749(07)01780-0

doi: 10.1016/j.jaci.2007.09.023

The Journal of Allergy and Clinical Immunology
Volume 120, Issue 6 , Pages 1301-1307 , December 2007

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