Genetic effect of CCR3 and IL5RA gene polymorphisms on eosinophilia in asthmatic patients

Gene map (A), linkage disequilibrium (LD) coefficients (B), and haplotypes (C) in CCR3. In the map (Fig 1, A), coding exons are marked by solid blocks and UTR by open blocks. The frequencies of SNPs were based on sequencing data (n = 24). The first base of the translation site was denoted as nucleotide +1.

The comparison of the number of eosinophils in peripheral blood between the subjects possessing ht1, ht2, or both of CCR3. The bars and error bars represent geometric means and 95% CIs, respectively. P values were obtained by means of linear regression analysis, controlling age and sex as covariates in the codominant model.

The CCR3 and IL-5Rα expression on the peripheral blood eosinophils from asthmatic patients. A, Gating for eosinophils among peripheral blood granulocytes by using autofluorescence is shown. B and D, Levels of CCR3 expression were compared between eosinophils from asthmatic patients having ht2 and having other haplotypes. C and E, Levels of IL-5Rα expression were compared between eosinophils from asthmatic patients who were G or A allele homozygotes on IL5RA-5091G>A. Each datum was presented by the ratio of fluorescence intensities for each molecule to the values of isotype-matched control.