The Journal of Allergy and Clinical Immunology
Volume 120, Issue 6 , Pages 1382-1388 , December 2007

Impaired immune response to vaccinia virus inoculated at the site of cutaneous allergic inflammation

  • Jordan E. Scott, MD

      Affiliations

    • Department of Pediatrics, Division of Allergy and Immunology, Children's Hospital, Harvard Medical School, Boston, Mass
    • These authors contributed equally to this work.
    • ,
  • Abdallah ElKhal, PhD

      Affiliations

    • Department of Pediatrics, Division of Allergy and Immunology, Children's Hospital, Harvard Medical School, Boston, Mass
    • These authors contributed equally to this work.
    • ,
  • Eva-Jasmin Freyschmidt, PhD

      Affiliations

    • Department of Pediatrics, Division of Allergy and Immunology, Children's Hospital, Harvard Medical School, Boston, Mass
    • ,
  • Daniel H. MacArthur, BS

      Affiliations

    • Department of Pediatrics, Division of Allergy and Immunology, Children's Hospital, Harvard Medical School, Boston, Mass
    • ,
  • Douglas McDonald, MD, PhD

      Affiliations

    • Department of Pediatrics, Division of Allergy and Immunology, Children's Hospital, Harvard Medical School, Boston, Mass
    • ,
  • Michael D. Howell, PhD

      Affiliations

    • National Jewish Medical and Research Center, Denver, Colo
    • ,
  • Donald Y.M. Leung, MD, PhD

      Affiliations

    • National Jewish Medical and Research Center, Denver, Colo
    • ,
  • Amale Laouar, PhD

      Affiliations

    • Center for Blood Research, Institute for Biomedical Research, Harvard Medical School, Boston, Mass
    • ,
  • N. Manjunath, PhD

      Affiliations

    • Center for Blood Research, Institute for Biomedical Research, Harvard Medical School, Boston, Mass
    • ,
  • Teresa Bianchi, PhD

      Affiliations

    • Brigham and Women's Hospital and Department of Pediatrics and Dermatology, Harvard Medical School, Boston, Mass
    • ,
  • Marianne Boes, PhD

      Affiliations

    • Brigham and Women's Hospital and Department of Pediatrics and Dermatology, Harvard Medical School, Boston, Mass
    • ,
  • Hans C. Oettgen, MD, PhD

      Affiliations

    • Department of Pediatrics, Division of Allergy and Immunology, Children's Hospital, Harvard Medical School, Boston, Mass
    • ,
  • Raif S. Geha, MD

      Affiliations

    • Department of Pediatrics, Division of Allergy and Immunology, Children's Hospital, Harvard Medical School, Boston, Mass
    • Corresponding Author InformationReprint requests: Raif S. Geha, MD, Division of Immunology, Karp 10th floor, One Blackfan Street, Boston, MA 02115.

Received 9 April 2007 ,Revised 30 July 2007 ,Accepted 1 August 2007.

  • Image Result

    A, EC sensitization and VV infection protocol. B and C, Skin infiltration with CD4+ T cells and eosinophils and expression of TH2 and TH1 cytokines at the time of VV inoculation. D, Representative sat

    A, EC sensitization and VV infection protocol. B and C, Skin infiltration with CD4+ T cells and eosinophils and expression of TH2 and TH1 cytokines at the time of VV inoculation. D, Representative satellite skin lesion. E, Satellite lesions in mice inoculated with VV at OVA versus saline-sensitized sites. ∗P < .05. HPF, High-power field; Eos, eosinophils; SAL, saline; ns, not significant.

  • Image Result
    TH1 (A) and TH2 (B) cytokine production by splenocytes of mice inoculated with VV at EC sensitization sites. SAL, Saline.

    TH1 (A) and TH2 (B) cytokine production by splenocytes of mice inoculated with VV at EC sensitization sites. SAL, Saline.

  • Image Result
    Serum IgG2a and IgG1 anti-VV antibody levels in mice inoculated with VV at EC sensitization sites. SAL, Saline.

    Serum IgG2a and IgG1 anti-VV antibody levels in mice inoculated with VV at EC sensitization sites. SAL, Saline.

  • Image Result
    TH1 cytokine production (A) and serum anti-VV Ab levels (B) in EC sensitized mice inoculated with VV at a distant skin site. SAL, Saline.

    TH1 cytokine production (A) and serum anti-VV Ab levels (B) in EC sensitized mice inoculated with VV at a distant skin site. SAL, Saline.

  • Image Result
    TH1 cytokine production (A) and serum anti-VV Ab levels (B) in EC sensitized mice inoculated intraperitoneally with VV. SAL, Saline.

    TH1 cytokine production (A) and serum anti-VV Ab levels (B) in EC sensitized mice inoculated intraperitoneally with VV. SAL, Saline.

  • Image Result
    Expression of CRAMP (A), IFN-α (B), and IFN-γ (C) mRNA in EC sensitized skin before and VV infection at the sensitization site. ∗P < .05. SAL, Saline.

    Expression of CRAMP (A), IFN-α (B), and IFN-γ (C) mRNA in EC sensitized skin before and VV infection at the sensitization site. ∗P < .05. SAL, Saline.

 Supported by federal funds from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services, under contract number HHSN266200400030C (Atopic Dermatitis Vaccinia Network).

 Disclosure of potential conflict of interest: J. Scott has consulting arrangements with Alicare Medical Management, owns Pharmaceutical holder trust stock, and is on the speakers' bureau for Verus Pharmaceuticals. The rest of the authors have declared that they have no conflict of interest.

PII: S0091-6749(07)01457-1

doi: 10.1016/j.jaci.2007.08.004

The Journal of Allergy and Clinical Immunology
Volume 120, Issue 6 , Pages 1382-1388 , December 2007

Article Tools

* Image Usage & Resolution