The Journal of Allergy and Clinical Immunology
Volume 119, Issue 1 , Pages 206-212 , January 2007

Esophageal remodeling in pediatric eosinophilic esophagitis

  • Seema S. Aceves, MD, PhD

      Affiliations

    • From the Divisions of Allergy, Immunology
    • Pediatrics
    • Corresponding Author InformationReprint requests: Seema S. Aceves, MD, PhD, Division of Allergy, Immunology, MC-5114, Children's Hospital, San Diego, 3020 Children's Way, San Diego, CA 92123.
    • ,
  • Robert O. Newbury, MD

      Affiliations

    • Pathology
    • ,
  • Ranjan Dohil, MD

      Affiliations

    • Pediatrics
    • Gastroenterology
    • ,
  • John F. Bastian, MD

      Affiliations

    • From the Divisions of Allergy, Immunology
    • Pediatrics
    • ,
  • David H. Broide, MBChB

      Affiliations

    • From the Divisions of Allergy, Immunology
    • Medicine, Children's Hospital, San Diego, and the University of California, San Diego

Received 15 June 2006 ,Revised 5 October 2006 ,Accepted 10 October 2006.

  • Image Result

    Pediatric patients with EE have increased subepithelial fibrosis and TGF-β1. A-D, Trichrome stain of normal, GERD, EE esophageal biopsies and fibrosis score. E, Representative image of EE biopsy stain

    Pediatric patients with EE have increased subepithelial fibrosis and TGF-β1. A-D, Trichrome stain of normal, GERD, EE esophageal biopsies and fibrosis score. E, Representative image of EE biopsy stained for TGF-β1 (red; inset shows green nucleus). F, Quantitated TGF-β1–positive cells in normal patients, patients with GERD, and patients with EE. G-I, Eosinophils express MBP (red), TGF-β1 (green), or both (yellow). ∗∗P < .005.

  • Image Result
    Increased nuclear phosopho-SMAD2/3 in the esophagus of patients with EE. A-F, Representative image of phospho-SMAD2/3 staining in epithelial (EPI) and lamina propria cells in patients with GERD (arrow

    Increased nuclear phosopho-SMAD2/3 in the esophagus of patients with EE. A-F, Representative image of phospho-SMAD2/3 staining in epithelial (EPI) and lamina propria cells in patients with GERD (arrows show phopho-SMAD2/3–negative nuclei) and patients with EE. G-I, A subset of actively proliferating epithelial cells coexpress (yellow) MIB-1 (green) and phosopho-SMAD2/3 (red). J, Quantitation of phospho-SMAD2/3–positive cells in normal, GERD, and EE. ∗P < .05; ∗∗P < .005.

  • Image Result
    Increased vascularity and VCAM-1 in the subepithelial esophagus of patients with EE. A-C, Representative images of vWF-positive vessels in the lamina propria of normal patients, patients with GERD, an

    Increased vascularity and VCAM-1 in the subepithelial esophagus of patients with EE. A-C, Representative images of vWF-positive vessels in the lamina propria of normal patients, patients with GERD, and patients with EE (inset shows blue nuclei). D and E, Quantitation of vWF and VCAM-positive vessels. ∗P < .05; ∗∗P < .005.

 Supported by the American Academy of Allergy, Asthma & Immunology Education and Research Trust (ERT) Clinical Research Grant (S.S.A.), National Institutes of Health T32 Training award AI 007469 (S.S.A.), and National Institutes of Health grant AI 38425 (D.H.B.).Disclosure of potential conflict of interest: S. S. Aceves has received grant support from the American Academy of Allergy, Asthma and Immunology Clinical ERT. D. H. Broide has received grant support from the National Institutes of Health. The other authors have declared that they have no conflict of interest.

PII: S0091-6749(06)02136-1

doi: 10.1016/j.jaci.2006.10.016

The Journal of Allergy and Clinical Immunology
Volume 119, Issue 1 , Pages 206-212 , January 2007

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