The Journal of Allergy and Clinical Immunology
Volume 116, Issue 2 , Pages 305-311 , August 2005

Dissecting asthma using focused transgenic modeling and functional genomics

  • Douglas A. Kuperman, PhD

      Affiliations

    • From the Department of Medicine, Allergy-Immunology Division, Northwestern University Feinberg School of Medicine, Chicago
    • Department of Medicine
    • Lung Biology Center
    • ,
  • Christina C. Lewis, PhD

      Affiliations

    • Department of Medicine
    • Lung Biology Center
    • ,
  • Prescott G. Woodruff, MD

      Affiliations

    • Department of Medicine
    • Division of Pulmonary and Critical Care Medicine
    • Cardiovascular Research Institute
    • ,
  • Madeleine W. Rodriguez, BS

      Affiliations

    • Department of Medicine
    • Lung Biology Center
    • ,
  • Yee Hwa Yang, PhD

      Affiliations

    • Department of Medicine
    • Lung Biology Center
    • ,
  • Gregory M. Dolganov, PhD

      Affiliations

    • Department of Medicine
    • Division of Pulmonary and Critical Care Medicine
    • Cardiovascular Research Institute
    • ,
  • John V. Fahy, MD

      Affiliations

    • Department of Medicine
    • Division of Pulmonary and Critical Care Medicine
    • Cardiovascular Research Institute
    • ,
  • David J. Erle, MD

      Affiliations

    • Department of Medicine
    • Lung Biology Center
    • Division of Pulmonary and Critical Care Medicine
    • Cardiovascular Research Institute
    • Program in Immunology, University of California San Francisco School of Medicine
    • Corresponding Author InformationReprint requests: David J. Erle, MD, UCSF Box 2922, San Francisco, CA 94143-2922.

Received 6 January 2005 ,Revised 28 February 2005 ,Accepted 9 March 2005.

  • Image Result

    Lung gene expression changes in 3 mouse asthma models. Differentially expressed genes were arranged by hierarchical clustering. Each column represents data from 1 of 5 individual mice in each group. C

    Lung gene expression changes in 3 mouse asthma models. Differentially expressed genes were arranged by hierarchical clustering. Each column represents data from 1 of 5 individual mice in each group. Colors represent fold-change compared with the appropriate controls. The arrow indicates a small group of genes that were increased in all 3 models. Ova, Ovalbumin.

  • Image Result
    Gene expression patterns. Grouping revealed genes with increased expression in (A) the ovalbumin (Ova) model only, (B) the Ova and tg–IL-13 models, (C) the tg–IL-13 model only, and (D) all 3 models. T

    Gene expression patterns. Grouping revealed genes with increased expression in (A) the ovalbumin (Ova) model only, (B) the Ova and tg–IL-13 models, (C) the tg–IL-13 model only, and (D) all 3 models. The number of genes (left) and representative genes (right) is shown for each group. Phenotypic attributes of each model are shown at the bottom. AHR, Airway hyperreactivity.

  • Image Result
    Intelectin (Itln) expression in human airway epithelial cells. A, Intelectin gene expression by airway epithelial cells from control subjects and subjects with asthma. Medians and interquartile ranges

    Intelectin (Itln) expression in human airway epithelial cells. A, Intelectin gene expression by airway epithelial cells from control subjects and subjects with asthma. Medians and interquartile ranges are shown. B, IL-13 treatment of cultured human airway epithelial cells induced increased expression of intelectin transcripts.

 Supported by National Institute of Health grants HL56835 and HL72301 and by the UCSF Sandler Center for Basic Research in Asthma.Disclosure of potential conflict of interest: D. A. Kuperman, none disclosed. C. A. Lewis, none disclosed. P. G. Woodruff, none disclosed. M. W. Rodriguez, none disclosed. Y. H. Yang, none disclosed. G. M. Dolganov, none disclosed. J. V. Fahy, none disclosed. D. J. Erle, none disclosed.

PII: S0091-6749(05)00598-1

doi: 10.1016/j.jaci.2005.03.024

The Journal of Allergy and Clinical Immunology
Volume 116, Issue 2 , Pages 305-311 , August 2005

Article Tools

* Image Usage & Resolution