The Journal of Allergy and Clinical Immunology
Volume 116, Issue 1 , Pages 73-79 , July 2005

IL-9 and c-Kit+ mast cells in allergic rhinitis during seasonal allergen exposure: Effect of immunotherapy

  • Kayhan T. Nouri-Aria, PhD, FRCPath

      Affiliations

    • These authors contributed equally to the present study.
    • ,
  • Charles Pilette, MD, PhD

      Affiliations

    • These authors contributed equally to the present study.
    • ,
  • Mikila R. Jacobson, PhD
    • ,
  • Hiroshi Watanabe, MD
    • ,
  • Stephen R. Durham, MD, FRCP

      Affiliations

    • Corresponding Author InformationReprint request: Stephen R. Durham, MD, FRCP, Upper Respiratory Medicine, Imperial College London, National Heart and Lung Institute, Dovehouse St, London SW3 6LY, United Kingdom.

Received 3 December 2004 ,Revised 4 March 2005 ,Accepted 9 March 2005.

  • Image Result

    Effects of natural allergen exposure and allergen immunotherapy on IL-9 protein (A), IL-9 mRNA (B), and c-Kit (C) expression in the nasal mucosa. Data shown are for out-of-season (pre) and peak-season

    Effects of natural allergen exposure and allergen immunotherapy on IL-9 protein (A), IL-9 mRNA (B), and c-Kit (C) expression in the nasal mucosa. Data shown are for out-of-season (pre) and peak-season (post) nasal biopsy specimens in immunotherapy- and placebo-treated patients and nonatopic control subjects (peak only). Horizontal bars represent median values.

  • Image Result
    Expression of IL-9 protein (immunohistochemistry [IMH], 200× magnification; a, IL-9 mRNA (in situ hybridization [ISH], 200× magnification; b, c-Kit+ mast cells (IMH, 200× magnification; c, and colocal

    Expression of IL-9 protein (immunohistochemistry [IMH], 200× magnification; a, IL-9 mRNA (in situ hybridization [ISH], 200× magnification; b, c-Kit+ mast cells (IMH, 200× magnification; c, and colocalization of IL-9 mRNA to CD3+ T lymphocytes (IMH/ISH, 400× magnification; d, and colocalization of IL-9 protein to CD3+ T lymphocytes (double immunofluorescence, 400× magnification; e, and IL-9 protein to MBP+ eosinophils (double immunofluorescence, 400× magnification; f, Arrows show IL-9–expressing cells.

  • Image Result
    The contributions to IL-9 protein expression by T cells (CD3), eosinophils (major basic protein [MBP]), neutrophils (Elastase), and mast cells (Tryptase) are shown (A), and the proportions of T cells,

    The contributions to IL-9 protein expression by T cells (CD3), eosinophils (major basic protein [MBP]), neutrophils (Elastase), and mast cells (Tryptase) are shown (A), and the proportions of T cells, eosinophils, neutrophils, and mast cells expressing IL-9 protein are shown (B). Contributions to IL-9 mRNA expression and proportions of IL-9 mRNA–expressing leukocytes are also shown (C and D, respectively).

 CP was supported by a research fellowship from the European Respiratory Society (Grant LTRF2002-037). This work was supported by the National Asthma Campaign (UK), Medical Research Council (UK), and ALK Abello (Horsholm, Denmark).Disclosure of potential conflict of interest: C. Pilette is supported by a European Respiratory Society (ERS) fellowship. S. Durham has consultant arrangements with and has received grants–research support from ALK-Abello and is on the speakers' bureau for ALK-Abello, GalxoSmithKline, Aventis, and UCB. All other authors—none disclosed.

PII: S0091-6749(05)00541-5

doi: 10.1016/j.jaci.2005.03.011

The Journal of Allergy and Clinical Immunology
Volume 116, Issue 1 , Pages 73-79 , July 2005

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