The Journal of Allergy and Clinical Immunology
Volume 122, Issue 4 , Pages 788-794, October 2008

A differential effect of 2 probiotics in the prevention of eczema and atopy: A double-blind, randomized, placebo-controlled trial

  • Kristin Wickens, PhD

      Affiliations

    • Wellington Asthma Research Group, Wellington School of Medicine and Health Sciences, University of Otago, Wellington, New Zealand
    • Corresponding Author InformationReprint requests: Kristin Wickens, PhD, Wellington Asthma Research Group, Wellington School of Medicine and Health Sciences, University of Otago, P O Box 7343, Wellington South, Wellington, New Zealand.
  • ,
  • Peter N. Black, FRACP

      Affiliations

    • Department of Pharmacology and Clinical Pharmacology, University of Auckland, Auckland, New Zealand
  • ,
  • Thorsten V. Stanley, FRCP

      Affiliations

    • Department of Paediatrics, Wellington School of Medicine and Health Sciences, University of Otago, Wellington, New Zealand
  • ,
  • Edwin Mitchell, FRACP, DSc

      Affiliations

    • Department of Paediatrics, University of Auckland, Auckland, New Zealand
  • ,
  • Penny Fitzharris, FRACP

      Affiliations

    • Immunology Department, Auckland Hospital, Auckland, New Zealand
  • ,
  • Gerald W. Tannock, PhD

      Affiliations

    • Department of Microbiology and Immunology, University of Otago, Wellington, New Zealand
  • ,
  • Gordon Purdie, BSc

      Affiliations

    • Department of Public Health, Wellington School of Medicine and Health Sciences, University of Otago, Wellington, New Zealand
  • ,
  • Julian Crane, FRACP

      Affiliations

    • Wellington Asthma Research Group, Wellington School of Medicine and Health Sciences, University of Otago, Wellington, New Zealand
  • ,
  • Probiotic Study Group

Received 9 April 2008; received in revised form 3 July 2008; accepted 14 July 2008. published online 02 September 2008.

Background

The role of probiotics in prevention of allergic disease is still not clearly established, although early reports suggested Lactobacillus GG halved the risk of eczema at 2 years.

Objective

To determine whether probiotic supplementation in early life could prevent development of eczema and atopy at 2 years.

Methods

Double-blind, randomized placebo-controlled trial of infants at risk of allergic disease. Pregnant women were randomized to take Lactobacillus rhamnosus HN001 (L rhamnosus), Bifidobacterium animalis subsp lactis strain HN019 or placebo daily from 35 weeks gestation until 6 months if breast-feeding, and their infants were randomized to receive the same treatment from birth to 2 years (n = 474). The infant's cumulative prevalence of eczema and point prevalence of atopy, using skin prick tests to common allergens, was assessed at 2 years.

Results

Infants receiving L rhamnosus had a significantly (P = .01) reduced risk of eczema (hazard ratio [HR], 0.51; 95% CI, 0.30-0.85) compared with placebo, but this was not the case for B animalis subsp lactis (HR, 0.90; 95% CI, 0.58-1.41). There was no significant effect of L rhamnosus (HR, 0.74; 95% CI, 0.46-1.18) or B animalis subsp lactis (HR, 0.82; 95% CI, 0.52-1.28) on atopy. L rhamnosus (71.5%) was more likely than B animalis subsp lactis (22.6%) to be present in the feces at 3 months, although detection rates were similar by 24 months.

Conclusion

We found that supplementation with L rhamnosus, but not B animalis subsp lactis, substantially reduced the cumulative prevalence of eczema, but not atopy, by 2 years. Understanding how Lactobacilli act to prevent eczema requires further investigation.

Key words: Probiotics, eczema, atopy, allergic disease, infants, allergy prevention, randomized controlled trial

Abbreviations used: HR, Hazard ratio, OR, Odds ratio, SCORAD, SCORing Atopic Dermatitis, SPT, Skin prick test

 

 Supported by the Health Research Council of New Zealand and Fonterra Co-operative Group (Auckland, New Zealand). Fonterra Co-operative Group provided the study capsules and maintained quality control of the products throughout the study. All other aspects of the study were conducted independently of Fonterra Co-operative Group.

 Disclosure of potential conflict of interest: K. Wickens has received research funding from the Health Research Council and Fonterra Co-operative Group. P. N. Black has received research funding from the Health Research Council of New Zealand, the Child Health Research Foundation, Fonterra Co-operative Group, and Boehringer-Ingelheim. T. V. Stanley has received research funding from the Health Research Council of New Zealand, Fonterra Co-operative Group, the Asthma and Respiratory Foundation of New Zealand, and the Wellington Medical Research Foundation. E. Mitchell has received research funding from the Child Health Research Foundation and the National Heart Foundation of New Zealand and has served as trustee in the past year for the Wilson & Sweet Trust and the Child Health Research Foundation. P. Fitzharris has received research funding from the Marsden Fund and the Foundation for Research, Science & Technology. G. W. Tannock has received research support from the Marsden Fund and the Foundation for Research, Science, and Technology. G. Purdie has received research funding from the Health Research Council of New Zealand, Fonterra Co-operative Group, and the Ministry of Health. J. Crane has received research funding from the Health Research Council of New Zealand, Fonterra Co-operative Group, Rex Medical, the Asthma and Respiratory Foundation of New Zealand, and the National Institute of Environmental Health Sciences.

PII: S0091-6749(08)01319-5

doi:10.1016/j.jaci.2008.07.011

The Journal of Allergy and Clinical Immunology
Volume 122, Issue 4 , Pages 788-794, October 2008