The Journal of Allergy and Clinical Immunology
Volume 116, Issue 2 , Pages 305-311 , August 2005

Dissecting asthma using focused transgenic modeling and functional genomics

  • Douglas A. Kuperman, PhD

      Affiliations

    • From the Department of Medicine, Allergy-Immunology Division, Northwestern University Feinberg School of Medicine, Chicago
    • Department of Medicine
    • Lung Biology Center
    • ,
  • Christina C. Lewis, PhD

      Affiliations

    • Department of Medicine
    • Lung Biology Center
    • ,
  • Prescott G. Woodruff, MD

      Affiliations

    • Department of Medicine
    • Division of Pulmonary and Critical Care Medicine
    • Cardiovascular Research Institute
    • ,
  • Madeleine W. Rodriguez, BS

      Affiliations

    • Department of Medicine
    • Lung Biology Center
    • ,
  • Yee Hwa Yang, PhD

      Affiliations

    • Department of Medicine
    • Lung Biology Center
    • ,
  • Gregory M. Dolganov, PhD

      Affiliations

    • Department of Medicine
    • Division of Pulmonary and Critical Care Medicine
    • Cardiovascular Research Institute
    • ,
  • John V. Fahy, MD

      Affiliations

    • Department of Medicine
    • Division of Pulmonary and Critical Care Medicine
    • Cardiovascular Research Institute
    • ,
  • David J. Erle, MD

      Affiliations

    • Department of Medicine
    • Lung Biology Center
    • Division of Pulmonary and Critical Care Medicine
    • Cardiovascular Research Institute
    • Program in Immunology, University of California San Francisco School of Medicine
    • Corresponding Author InformationReprint requests: David J. Erle, MD, UCSF Box 2922, San Francisco, CA 94143-2922.

Received 6 January 2005 ,Revised 28 February 2005 ,Accepted 9 March 2005.

References 

  1. Bousquet J, Jeffery PK, Busse WW, Johnson M, Vignola AM. Asthma: from bronchoconstriction to airways inflammation and remodeling. Am J Respir Crit Care Med. 2000;161:1720–1745
  2. Laprise C, Sladek R, Ponton A, Bernier MC, Hudson TJ, Laviolette M. Functional classes of bronchial mucosa genes that are differentially expressed in asthma. BMC Genomics. 2004;5:21
  3. Zimmermann N, King NE, Laporte J, Yang M, Mishra A, Pope SM, et al. Dissection of experimental asthma with DNA microarray analysis identifies arginase in asthma pathogenesis. J Clin Invest. 2003;111:1863–1874
  4. Zou J, Young S, Zhu F, Gheyas F, Skeans S, Wan Y, et al. Microarray profile of differentially expressed genes in a monkey model of allergic asthma. Genome Biol. 2002;3:research0020.1-0020.13
  5. Kuperman DA, Huang X, Koth LL, Chang GH, Dolganov GM, Zhu Z, et al. Direct effects of interleukin-13 on epithelial cells cause airway hyperreactivity and mucus overproduction in asthma. Nat Med. 2002;8:885–889
  6. Huang SK, Xiao HQ, Kleine-Tebbe J, Paciotti G, Marsh DG, Lichtenstein LM, et al. IL-13 expression at the sites of allergen challenge in patients with asthma. J Immunol. 1995;155:2688–2694
  7. Humbert M, Durham SR, Kimmitt P, Powell N, Assoufi B, Pfister R, et al. Elevated expression of messenger ribonucleic acid encoding IL-13 in the bronchial mucosa of atopic and nonatopic subjects with asthma. J Allergy Clin Immunol. 1997;99:657–665
  8. Grunig G, Warnock M, Wakil AE, Venkayya R, Brombacher F, Rennick DM, et al. Requirement for IL-13 independently of IL-4 in experimental asthma. Science. 1998;282:2261–2263
  9. Wills-Karp M, Luyimbazi J, Xu X, Schofield B, Neben TY, Karp CL, et al. Interleukin-13: central mediator of allergic asthma. Science. 1998;282:2258–2261
  10. Zhu Z, Homer RJ, Wang Z, Chen Q, Geba GP, Wang J, et al. Pulmonary expression of interleukin-13 causes inflammation, mucus hypersecretion, subepithelial fibrosis, physiologic abnormalities, and eotaxin production. J Clin Invest. 1999;103:779–788
  11. Nelms K, Keegan AD, Zamorano J, Ryan JJ, Paul WE. The IL-4 receptor: signaling mechanisms and biologic functions. Annu Rev Immunol. 1999;17:701–738
  12. Stripp BR, Sawaya PL, Luse DS, Wikenheiser KA, Wert SE, Huffman JA, et al. cis-acting elements that confer lung epithelial cell expression of the CC10 gene. J Biol Chem. 1992;267:14703–14712
  13. Barczak A, Rodriguez MW, Hanspers K, Koth LL, Tai YC, Bolstad BM, et al. Spotted long oligonucleotide arrays for human gene expression analysis. Genome Res. 2003;13:1775–1785
  14. Diaz E, Yang YH, Ferreira T, Loh KC, Okazaki Y, Hayashizaki Y, et al. Analysis of gene expression in the developing mouse retina. Proc Natl Acad Sci U S A. 2003;100:5491–5496
  15. Jin W, Riley RM, Wolfinger RD, White KP, Passador-Gurgel G, Gibson G. The contributions of sex, genotype and age to transcriptional variance in Drosophila melanogaster. Nat Genet. 2001;29:389–395
  16. Lonnstedt I, Speed TP. Replicated microarray data. Statistica Sinica. 2002;12:31–46
  17. Smyth GK. Linear models and empirical Bayes methods for assessing differential expression in microarray experiments. Stat Appl Genet Mol Biol. 2004;3:3;Available at: http://www.bepress.com/sagmb/vol3/iss1/art3/
  18. Claman DM, Boushey HA, Liu J, Wong H, Fahy JV. Analysis of induced sputum to examine the effects of prednisone on airway inflammation in asthmatic subjects. J Allergy Clin Immunol. 1994;94:861–869
  19. Chai H, Farr RS, Froehlich LA, Mathison DA, McLean JA, Rosenthal RR, et al. Standardization of bronchial inhalation challenge procedures. J Allergy Clin Immunol. 1975;56:323–327
  20. Atherton HC, Jones G, Danahay H. IL-13-induced changes in the goblet cell density of human bronchial epithelial cell cultures: MAP kinase and phosphatidylinositol 3-kinase regulation. Am J Physiol Lung Cell Mol Physiol. 2003;285:L730–L739
  21. Dolganov GM, Woodruff PG, Novikov AA, Zhang Y, Ferrando RE, Szubin R, et al. A novel method of gene transcript profiling in airway biopsy homogenates reveals increased expression of a Na+-K+-Cl- cotransporter (NKCC1) in asthmatic subjects. Genome Res. 2001;11:1473–1483
  22. Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, et al. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 2002;3:research0034.1-0034.11
  23. Westfall PH, Young SS. Resampling-based multiple testing: examples and methods for p-value adjustment. New York: John Wiley & Sons; 1993;
  24. Ge Y, Dudoit S, Speed TP. Resampling-based multiple testing for microarray data hypothesis. Test. 2003;12:1–44
  25. Hoshino M, Morita S, Iwashita H, Sagiya Y, Nagi T, Nakanishi A, et al. Increased expression of the human Ca2+-activated Cl- channel 1 (CaCC1) gene in the asthmatic airway. Am J Respir Crit Care Med. 2002;165:1132–1136
  26. Nakanishi A, Morita S, Iwashita H, Sagiya Y, Ashida Y, Shirafuji H, et al. Role of gob-5 in mucus overproduction and airway hyperresponsiveness in asthma. Proc Natl Acad Sci U S A. 2001;98:5175–5180
  27. Zhou Y, Shapiro M, Dong Q, Louahed J, Weiss C, Wan S, et al. A calcium-activated chloride channel blocker inhibits goblet cell metaplasia and mucus overproduction. Novartis Found Symp. 2002;248:150–165discussion 65-70, 277-82
  28. Johnson HG, McNee ML, Sun FF. 15-Hydroxyeicosatetraenoic acid is a potent inflammatory mediator and agonist of canine tracheal mucus secretion. Am Rev Respir Dis. 1985;131:917–922
  29. Copas JL, Borgeat P, Gardiner PJ. The actions of 5-, 12-, and 15-HETE on tracheobronchial smooth muscle. Prostaglandins Leukot Med. 1982;8:105–114
  30. Lai CK, Polosa R, Holgate ST. Effect of 15-(s)-hydroxyeicosatetraenoic acid on allergen-induced asthmatic responses. Am Rev Respir Dis. 1990;141:1423–1427
  31. Nikolaidis NM, Zimmermann N, King NE, Mishra A, Pope SM, Finkelman FD, et al. Trefoil factor-2 is an allergen-induced gene regulated by Th2 cytokines and STAT6 in the lung. Am J Respir Cell Mol Biol. 2003;29:458–464
  32. Oertel M, Graness A, Thim L, Buhling F, Kalbacher H, Hoffmann W. Trefoil factor family-peptides promote migration of human bronchial epithelial cells: synergistic effect with epidermal growth factor. Am J Respir Cell Mol Biol. 2001;25:418–424
  33. Farrell JJ, Taupin D, Koh TJ, Chen D, Zhao CM, Podolsky DK, et al. TFF2/SP-deficient mice show decreased gastric proliferation, increased acid secretion, and increased susceptibility to NSAID injury. J Clin Invest. 2002;109:193–204
  34. Tsuji S, Uehori J, Matsumoto M, Suzuki Y, Matsuhisa A, Toyoshima K, et al. Human intelectin is a novel soluble lectin that recognizes galactofuranose in carbohydrate chains of bacterial cell wall. J Biol Chem. 2001;276:23456–23463
  35. Sharma P, Dudus L, Nielsen PA, Clausen H, Yankaskas JR, Hollingsworth MA, et al. MUC5B and MUC7 are differentially expressed in mucous and serous cells of submucosal glands in human bronchial airways. Am J Respir Cell Mol Biol. 1998;19:30–37
  36. Zhu Z, Zheng T, Homer RJ, Kim YK, Chen NY, Cohn L, et al. Acidic mammalian chitinase in asthmatic Th2 inflammation and IL-13 pathway activation. Science. 2004;304:1678–1682

 Supported by National Institute of Health grants HL56835 and HL72301 and by the UCSF Sandler Center for Basic Research in Asthma.Disclosure of potential conflict of interest: D. A. Kuperman, none disclosed. C. A. Lewis, none disclosed. P. G. Woodruff, none disclosed. M. W. Rodriguez, none disclosed. Y. H. Yang, none disclosed. G. M. Dolganov, none disclosed. J. V. Fahy, none disclosed. D. J. Erle, none disclosed.

PII: S0091-6749(05)00598-1

doi: 10.1016/j.jaci.2005.03.024

The Journal of Allergy and Clinical Immunology
Volume 116, Issue 2 , Pages 305-311 , August 2005

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