The Journal of Allergy and Clinical Immunology
Volume 112, Issue 2 , Pages 438-444 , August 2003

The −159 C→T polymorphism of CD14 is associated with nonatopic asthma and food allergy

  • Jessica G. Woo, MHSA

      Affiliations

    • the Division of Epidemiology and Biostatistics, Department of Environmental Health, University of Cincinnati Medical Center Cincinnati, Ohio
    • ,
  • Amal Assa’ad, MD

      Affiliations

    • the Division of Allergy and Immunology, Department of Pediatrics, Cincinnati Children's Hospital Medical Center Cincinnati, Ohio
    • ,
  • Angela B. Heizer, BA

      Affiliations

    • the Division of Allergy and Immunology, Department of Pediatrics, Cincinnati Children's Hospital Medical Center Cincinnati, Ohio
    • ,
  • Jonathan A. Bernstein, MD

      Affiliations

    • the Division of Immunology, Department of Internal Medicine, University of Cincinnati Medical Center. Cincinnati, Ohio
    • ,
  • Gurjit K. Khurana Hershey, MD, PhD

      Affiliations

    • the Division of Allergy and Immunology, Department of Pediatrics, Cincinnati Children's Hospital Medical Center Cincinnati, Ohio

Received 8 January 2003 ,Revised 1 May 2003 ,Accepted 2 May 2003.

  • Image Result

    Identification of −159 C and −159 T CD14 allelic variants. Patients were genotyped with a PCR-based RFLP assay (see the “Methods” section). The C allele is uncut at 497 bp, whereas the T allele is cut

    Identification of −159 C and −159 T CD14 allelic variants. Patients were genotyped with a PCR-based RFLP assay (see the “Methods” section). The C allele is uncut at 497 bp, whereas the T allele is cut, resulting in bands of 144 and 353 bp. Lane 1, 100-bp ladder; lanes 2-7, genotypes noted above lane.

☆ Supported by NIH R01AI46652-01A1 (Dr Hershey). Dr Woo was supported by a National Institute of Environmental Health Sciences' Molecular Epidemiology in Children's Environmental Health Training Grant, T32-ES 10957.

☆☆ Reprint requests: Gurjit K. Khurana Hershey, MD, PhD, Division of Allergy and Immunology, Children's Hospital Medical Center, 3333 Burnet Ave, Cincinnati, OH 45229.

PII: S0091-6749(03)01601-4

doi: 10.1067/mai.2003.1634

The Journal of Allergy and Clinical Immunology
Volume 112, Issue 2 , Pages 438-444 , August 2003

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